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Innate trypanolytic factors in triatomine hemolymph against Trypanosoma rangeli and T. cruzi: a comparative study in eight Chagas disease vectors

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dc.contributor.authorSuárez Quevedo, Yazmin
dc.contributor.authorBarbosa Vinasco, Hamilton J.
dc.contributor.authorGutiérrez Garnizo, Sneider A.
dc.contributor.authorOlaya Morales, Jenny L.
dc.contributor.authorZabala González, Daniel
dc.contributor.authorCarranza Martínez, Julio C.
dc.contributor.authorGuhl Nannetti, Felipe
dc.contributor.authorCantillo Barraza, Omar
dc.contributor.authorVallejo, Gustavo A.
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.date.accessioned2021-12-10T08:07:57Z
dc.date.available2021-12-10T08:07:57Z
dc.date.issued2020-03-25
dc.description.abstractExiste información limitada sobre la inmunidad innata en triatominos contra Trypanosoma rangeli, un parásito humano, infeccioso y no patogénico, y T. cruzi, el agente casal de la enfermedad de Chagas. Sin embargo, esta información podría ayudar a identificar los factores que afectan la transmisión de los parásitos. Por lo tanto, nuestro objetivo fue abordar este vacío mediante el estudio de la actividad tripanolítica in vitro en la hemolinfa de insectos no infectados con T. rangeli o T. cruzi. Se examinaron ocho especies de triatominos, incluyendo Rhodnius prolixus, R. robustus, R. colombiensis, R. pallescens, R. pictipes, Triatoma dimidiata, T. maculata, and Panstrongylus geniculatus. La hemolinfa de las colonias de laboratorio de R. prolixus y R. robustus demostró una fuerte actividad tripanolítica contra algunos genotipos de T. rangeli y T. cruzi durante las primeras 14 h de incubación. Sin embargo, no se detectó actividad lítica a las 14 o 24 h usando hemolinfa de colonias de laboratorio de R. pallescens, R. pictipes, T. maculata y P. geniculatus, o de colonias de laboratorio o poblaciones silvestres de R. colombiensis y T. dimidiata. Este es el primer estudio comparativo de la actividad tripanolítica en la hemolinfa de diferentes especies de triatominos contra varios genotipos de T. rangeli y T. cruzi. Aunque las estructuras químicas de estos factores líticos, junto con los mecanismos que determinan su expresión en los insectos, no se han determinado completamente, su identificación permitirá nuestra comprensión de la inmunidad innata de los triatominos y su papel en la transmisión de los tripanosomas.spa
dc.description.abstractThere is limited information about the innate immunity of triatomines against Trypanosoma rangeli, an infectious, non-pathogenic human parasite, and T. cruzi, the causative agent of Chagas’ disease. This study aimed at addressing this gap by studying the in vitro trypanolytic hemolymph activity from insects not infected by T. rangeli or T. cruzi. Eight triatomine species were examined including Rhodnius prolixus, R. robustus, R. colombiensis, R. pallescens, R. pictipes, Triatoma dimidiata, T. maculata, and Panstrongylus geniculatus. The hemolymph of R. prolixus and R. robustus laboratory colonies demonstrated strong trypanolytic activity during the first 14 hours of parasite incubation with 81% lysis for the T. rangeli C genotype, 90% for the E genotype, 95% for T. cruzi discrete taxonomic unit (DTU) TcII, 94% for TcV, 96% for TcVI, 94% for Tcbat, and 90% for Tcmarinkellei. No lysis activity was detected 14 or 24 hours after parasite incubation with the hemolymph of insects from R. pictipes R. pallescens, P. geniculatus, and T. maculata colonies. Identical results (absence of lysis) were observed using hemolymph from R. colombiensis and T. dimidiata laboratory colonies and specimens captured in sylvatic environments. The hemolymph lytic activity against recently obtained cultures (95% decrease in live parasite count) and long-term T. cruzi TcII ones (96% decrease) was similar. Lytic activity was similar in hemolymph from R. prolixus nymphs, males and females and insects fed on chicken or mouse blood. This is the first comparative study of the trypanolytic activity of hemolymph from different triatomine species against T. rangeli and T. cruzi genotypes. Although the chemical structures of such lysis factors and the mechanisms determining their expression have not been fully determined, their identification furthers our understanding of triatomines’ innate immunity and their role in Trypanosoma transmission.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.18257/raccefyn.1097
dc.identifier.urihttps://repositorio.accefyn.org.co/handle/001/1184
dc.language.isospaspa
dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.publisher.placeBogotá, Colombiaspa
dc.relation.citationendpage104spa
dc.relation.citationissue170spa
dc.relation.citationstartpage88spa
dc.relation.citationvolume44spa
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourceRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.subject.proposalTrypanosoma cruzispa
dc.subject.proposalTrypanosoma cruzieng
dc.subject.proposalTrypanosoma rangelispa
dc.subject.proposalTrypanosoma rangelieng
dc.subject.proposalRhodnius prolixusspa
dc.subject.proposalRhodnius prolixuseng
dc.subject.proposalRhodnius robustusspa
dc.subject.proposalRhodnius robustuseng
dc.subject.proposalFactores tripanolíticosspa
dc.subject.proposalTrypanolytic factorseng
dc.subject.proposalCapacidad vectorialspa
dc.subject.proposalVectorial abilityeng
dc.subject.proposalInmunidad en insectosspa
dc.subject.proposalInsect immunityeng
dc.titleInnate trypanolytic factors in triatomine hemolymph against Trypanosoma rangeli and T. cruzi: a comparative study in eight Chagas disease vectorsspa
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentDataPaperspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombianaspa
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