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dc.contributor.authorQuintero Mercado, Andrés-
dc.contributor.authorDangon Bernier, Fabio-
dc.contributor.authorPáez Redondo, Alberto-
dc.date.accessioned2021-12-09T21:48:02Z-
dc.date.available2021-12-09T21:48:02Z-
dc.date.issued2019-04-09-
dc.identifier.urihttps://repositorio.accefyn.org.co/handle/001/1123-
dc.description.abstractLa antracnosis es la enfermedad más limitante en cultivos de mango en el departamento del Magdalena, Colombia, pues causa pérdidas de 40 a 50 % de la cosecha. A nivel mundial, se registran pérdidas en campo hasta de 51 % y en poscosecha, de 20 a 30 %. El agente causal, Colletotrichum spp., es un hemibiótrofo que inicialmente no produce síntomas en su hospedante, pero después se torna necrotrófico. El endofitismo es un estilo de vida del hongo cuyo papel en la patogenia es aún incierto. En este contexto, se propuso la caracterización de aislamientos endofíticos de Colletotrichum spp. a partir de hojas y ramas de mango cultivar Azúcar de un cultivo ubicado en Ciénaga. Se determinó la prevalencia de los aislamientos en hojas y ramas de tres estratos del árbol mediante tres protocolos de extracción: 1) en cámara de cloro, 2) con hipoclorito de sodio al 4 %, y 3) con hipoclorito de sodio al 2 %. Se describió la morfometría (colonia, conidios y apresorios), se hizo la prueba de patogenia en los frutos y la identificación molecular del aislamiento más virulento. Se obtuvieron nueve cepas endofíticas, ocho de hojas y una de ramas. La mejor extracción (18,2 %) se logró con el hipoclorito de sodio al 2 %; la prevalencia fue mayor en el estrato superior (10 % de las muestras) y en las hojas (8,9 % de las muestras). Las cepas endofíticas indujeron lesiones de 0,65 a 19,7 mm de diámetro y la más virulenta (HP3A-4) se identificó molecularmente como C. tropicale, siendo este el primer registro en Colombia del hongo como endófito patogénico en el mango. Se concluyó que es posible encontrar Colletotrichum como endófito en hojas y ramas de mango y que puede causar infección al inocularse en los frutos, evidenciando así una gran probabilidad de que actúe como un inóculo oculto para el desarrollo de la antracnosis en el mango.spa
dc.description.abstractAnthracnose is the most limiting disease in mango crops in the department of Magdalena (Colombia), causing crop losses between 40 and 50%; worldwide, losses in the field of up to 51% of the crop have been reported and between 20 and 30% in postharvest. The causal agent, Colletotrichum spp., has a hemibiotrophic lifestyle, initially does not induce symptoms in its host, but later it changes to a necrotrophic stage. Endophytism is a lifestyle of Colletotrichum, but its role in pathogenicity is still uncertain, which led us to characterize endophytic isolates of Colletotrichum spp. from leaves and branches of mango, cultivar Azúcar, from a crop located in Ciénaga (Magdalena). The prevalence of the isolates in leaves and branches was determined considering three strata of the tree using three extraction protocols: (1) Chlorine chamber; (2) 4% sodium hypochlorite, and (3) 2% sodium hypochlorite. Morphometric descriptions included the colony, the conidia, and the appressoria. We tested pathogenicity in fruits and carried out the molecular identification of the most pathogenic isolates. We obtained nine endophytic isolates, eight in leaves and one in branches. The best extraction (18.2%) protocol was the one using 2% sodium hypochlorite. The highest prevalence was found in the upper strata of the tree (10% of the samples) and in the leaves (8.9% of the samples). The endophytic strains induced lesions diameter between 0,65-19,7 mm in diameter in fruits. The most virulent strain (HP3A-4) was identified as C. tropicale by molecular techniques. This is the first report in Colombia of this fungus acting as pathogenic endophyte on mango. The present study indicated that it is possible to find Colletotrichum as an endophyte in mango leaves and branches, which can cause infection when inoculated in fruits and evidences its high hidden inoculum potential for the development of anthracnosis.eng
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dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.sourceRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.titleAislamientos endofíticos de Colletotrichum spp. a partir de hojas y ramas de mango (Mangifera indica L.) cultivar Azúcar en el municipio de Ciénaga, Magdalena, Colombiaspa
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombianaspa
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dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.identifier.doihttps://doi.org/10.18257/raccefyn.788-
dc.subject.proposalHemibiotrofospa
dc.subject.proposalHemibiotrophiceng
dc.subject.proposalPrevalenciaspa
dc.subject.proposalPrevalenceeng
dc.subject.proposalPatogenicidadspa
dc.subject.proposalPathogenicityeng
dc.subject.proposalInóculo ocultospa
dc.subject.proposalHidden inoculumeng
dc.subject.proposalC. tropicalespa
dc.subject.proposalC. tropicaleeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.relation.citationvolume43spa
dc.relation.citationstartpage65spa
dc.relation.citationendpage77spa
dc.publisher.placeBogotá, Colombiaspa
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.coverage.regionCiénaga, Magdalena, Colombia-
dc.relation.citationissue166spa
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