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dc.contributor.authorPinto, Sandra M.-
dc.contributor.authorPinzón, Edgar F.-
dc.contributor.authorMeléndez, Angel M.-
dc.contributor.authorMendez Sanchez, Stelia-
dc.contributor.authorMiranda, David A.-
dc.description.abstractLa espectroscopia de impedancia eléctrica (EIS) se emplea en el estudio de las propiedades eléctricas de células en suspensión. Para medir las propiedades eléctricas de materiales se utilizan dos (sistema de electrodo bipolar), tres (tripolares) y cuatro (tetrapolares) electrodos, siendo las medidas tetrapolares la mejor opción para minimizar los efectos de polarización de electrodo. Cuando se repiten las medidas de EIS en una misma muestra, se espera obtener espectros similares, sin embargo, en algunas situaciones se encuentran diferencias significativas asociadas con la baja reproducibilidad y repetibilidad. Aquí se utilizaron células HeLa dispersas en solución acuosa para evaluar también el efecto de la limpieza en el funcionamiento de los electrodos de oro. La limpieza de las superficies de los electrodos de oro se hizo por voltamperometría cíclica. Se encontró que la reproducibilidad y la repetibilidad de las medidas de impedancia aumentaba al limpiar los electrodos. Estos resultados evidenciaron que la limpieza de la superficie de los electrodos minimizó las contribuciones de la polarización de los electrodos en las mediciones. Además, se propone un modelo de circuito para describir el efecto de las superficies sin limpiar en las medidas eléctricas. Dicho modelo confirma que el efecto observado no se debió a una impedancia de polarización convencional. Más bien se trató de una polarización activa (como un potencial), la cual se presenta a bajas frecuencias y es independiente de la frecuencia de la señal de excitació
dc.description.abstractElectrical impedance spectroscopy (EIS) is used to study the electrical properties of cells in suspension. Two (bipolar electrode system), three (tripolar) and four (tetrapolar) electrodes are used to measure the electrical properties of materials with tetrapolar measurements being the best option to minimize electrode polarization effects. When EIS measurements are repeated for the same sample, almost identical spectra can be expected. However, in some situations, significant differences associated with low reproducibility and repeatability are found. Herein we used HeLa cells dispersed in an aqueous solution to evaluate the effect of cleaning on the performance of gold electrodes. The surface cleaning of gold electrodes was conducted by cyclic voltammetry. We found that the impedance measuring reproducibility and repeatability are enhanced when the electrodes are cleaned. These results show that surface cleaning minimizes the contribution of electrode polarization in measurements. Additionally, a circuital model is proposed to describe the effect of untreated surfaces on electrical measurement. The circuital model confirms that the observed effect is not due to a typical polarization impedance. Rather, it is an active polarization (interfacial polarization), which occurs at low frequencies, and is independent of the frequency of the excitation signal.eng
dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.sourceRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.titleElectrode cleaning and reproducibility of electrical impedance measurements of HeLa cells on aqueous solutionspa
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombianaspa
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dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.subject.proposalEspectroscopia de impedancia eléctricaspa
dc.subject.proposalElectrical impedance spectroscopyeng
dc.subject.proposalCéulas HeLaspa
dc.subject.proposalHeLa cellseng
dc.subject.proposalFenómenos de superficiespa
dc.subject.proposalSurface phenomenaeng
dc.subject.proposalModelo circuitalspa
dc.subject.proposalCircuital modeleng
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.publisher.placeBogotá, Colombiaspa
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
Appears in Collections:BA. Revista de la Academia Colombiana de Ciencias Exactas Físicas y Naturales

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