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dc.contributor.authorBenavides Serrato, Milena-
dc.contributor.authorContreras Rueda, Yully F.-
dc.contributor.authorBarrios Gardelis, Lina M.-
dc.contributor.authorFox, Graeme-
dc.contributor.authorHughes, Thomas D.-
dc.contributor.authorCampos Campos, Néstor H.-
dc.contributor.authorPreziosi, Richard-
dc.description.abstractComo primer paso para establecer la estructura genética del erizo de mar Echinometra lucunter lucunter a lo largo del Caribe, se aislaron 26 microsatélites usando secuenciación de extremo pareado (Next Generation Sequencing, NGS) Illumina. Se optimizaron exitosamente 17 marcadores y se probó su variación alélica en 23 individuos recolectados a lo largo del mar Caribe y en Cabo Verde, Atlántico oriental tropical. El número de alelos por locus (Na) fluctuó entre cuatro y 24, la heterocigosidad observada (Ho) entre 0,682 y 1 y la heterocigosidad esperada (He), entre 0,609 y 0,9304. No hubo desequilibrio de ligamento entre los pares de locus detectados. Los microsatelites aislados e identificados se usarán por primera vez para detectar la influencia de las barreras marinas en el flujo génico del erizo E. lucunter lucunter a lo largo del mar Caribe. Estos nuevos marcadores serán esenciales para la conservación y los estudios de conectividad a través del mar Caribe y el océano Atlántico, área donde se distribuye la
dc.description.abstractAs a first step to establish the genetic structure of the sea urchin Echinometra lucunter lucunter throughout the Caribbean Sea, 26 microsatellite loci were isolated using Illumina paired-end sequencing, Next Generation Sequencing (NGS). We successfully optimized 17 loci for genotyping and the variation tested for 23 individuals from the Caribbean Sea and Tropical Eastern Atlantic Ocean. The allele number per locus (Na) ranged from four to 24, the observed heterozygosity (Ho) from 0.682 to 1, and the expected heterozygosity (He) from 0.609 to 0.9304. We detected no linkage disequilibrium between pairs of loci. These microsatellites will be used for the first time to detect the influence of marine barriers to genetic flow in the sea urchin E. lucunter lucunter throughout the Caribbean Sea. These new validated markers will be essential for current conservation and connectivity studies across the Caribbean Sea and the Atlantic Ocean.eng
dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.sourceRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.titleIsolation and characterization of 17 polymorphic microsatellite loci for a sea urchin (Echinometra lucunter: Echinometridae)spa
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad científica colombianaspa
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dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.subject.proposalConectividad marinaspa
dc.subject.proposalMarine connectivityeng
dc.subject.proposalMar Caribespa
dc.subject.proposalCaribbean Seaeng
dc.subject.proposalMarcadores nuclearesspa
dc.subject.proposalNuclear markerseng
dc.subject.proposalEchinometra lucunterspa
dc.subject.proposalEchinometra lucuntereng
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.publisher.placeBogotá, Colombiaspa
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.coverage.regionMar Caribe-
Appears in Collections:BA. Revista de la Academia Colombiana de Ciencias Exactas Físicas y Naturales

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