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dc.contributor.authorGuarín, Paula-
dc.contributor.authorCristancho, Jonatan-
dc.contributor.authorCastillo, John J.-
dc.description.abstractSe desarrolló un novedoso método electroquímico para la detección rápida y analítica de Staphylococcus aureus en cultivos y muestras de leche enriquecidas con peróxido de hidrógeno (H2O2) mediante el consumo de péroxido por electrodos serigrafiados de oro (ESO) modificados con cisteína y peroxidasa de pasto Guinea (PPG). La peroxidasa de las hojas del pasto Guinea, con una actividad específica de 470 U mg-1, se inmovilizó sobre la superficie de los ESO previamente modificada con cisteína. Los voltamperogramas cíclicos de los ESO modificados con PPG y cisteína en presencia de ferrocianuro de potasio como sonda redox exhibieron un incremento en la corriente de aproximadamente 5 % comparado con el electrodo sin modificar. El ESO modificado mostró una buena respuesta electrocatalítica en la reducción de H2O2. El medio de cultivo en presencia de 1x10-3 M de H2O2 registró una disminución en la corriente por la catalasa presente en S. aureus a un potencial de -780 mV. El ESO modificado pudo detectar S. aureus en un rango de concentraciones entre 3x102 and 3x108 UFC mL-1 con un límite de detección de 102 ufc mL-1, un tiempo de detección de ~20 min y una sensibilidad de 0,020 mA
dc.description.abstractWe present a novel electrochemical method for a rapid analytical detection of Staphylococcus aureus in culture and spiked milk samples through hydrogen peroxide (H2O2) consumption on a screenprinted gold electrode (SPGE) modified with cysteine and peroxidase from Guinea grass leaves (GGP). This peroxidase (POD) had a specific activity of 470 U mg-1 and it was immobilized on an SPGE surface previously modified with cysteine. Cyclic voltammograms of gold electrodes modified with peroxidase and cysteine in the presence of potassium ferrocyanide as a redox probe demonstrated an increase of approximately 5% in the current compared to the bare gold electrode. The SPGE modified electrode exhibited a good electrocatalytic response towards H2O2 reduction. We added a constant H2O2 concentration of 1x10-3 M to the culture medium and measured the decrease in the H2O2 current at -780 mV consumed by catalase from S. aureus. Our modified electrode proved to sense S. aureus in a range concentration between 3x102 and 3x108 CFU/mL-1 with a detection limit of 102 CFU/mL-1, detection time of about ~20 min, and a sensitivity of 0.020 mA CFU-1.eng
dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.sourceRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.titleRapid electrochemical detection of Staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and Guinea grass (Panicum maximum) peroxidasespa
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad científicaspa
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dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.subject.proposalPeroxidasas de pasto guineaspa
dc.subject.proposalGuinea grass peroxidaseeng
dc.subject.proposalStaphylococcus aureusspa
dc.subject.proposalStaphylococcus aureuseng
dc.subject.proposalDetección electroquímicaspa
dc.subject.proposalElectrochemical detectioneng
dc.subject.proposalElectrodos serigrafiadosspa
dc.subject.proposalScreenprinted electrodes.eng
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.publisher.placeBogotá, Colombiaspa
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
Appears in Collections:BA. Revista de la Academia Colombiana de Ciencias Exactas Físicas y Naturales

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