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dc.contributor.authorGranados-Ramírez, Carmen Giovana-
dc.contributor.authorContreras-Rodríguez, Luis Ernesto-
dc.contributor.authorRamírez-Hernández, María Helena-
dc.date.accessioned2022-11-01T01:49:43Z-
dc.date.available2022-11-01T01:49:43Z-
dc.date.issued2021-09-16-
dc.identifier.issn0370-3908spa
dc.identifier.urihttps://repositorio.accefyn.org.co/handle/001/1985-
dc.description.abstractLa nicotinamida/nicotinato mononucleótido adenililtransferasa (NMNAT, EC 2.7.7.1/18) desempeña una función central en la síntesis del dinucleótido de adenina y nicotinamida (NAD) debido a que en esta enzima confluyen las rutas de síntesis de novo y de reciclaje. El NAD es una molécula trascendental en el metabolismo de todos los seres vivos, principalmente en el metabolismo redox. En este estudio se presenta una nueva estrategia metodológica para la evaluación de posibles inhibidores de la NMNAT de Leishmania braziliensis (LbNMNAT). El método suprime la actividad inhibitoria cruzada con la enzima acoplada al ensayo de detección, la alcohol dehidrogenasa (ADH, EC 1.1.1.1). Experimentalmente se introdujo un paso intermedio de extracción en fase sólida de los inhibidores antes de la ejecución del sistema enzimático de detección. La implementación del paso de extracción posibilitó la evaluación específica de la enzima de interés, la LbNMNAT, sin afectar la enzima acoplada ADH. El nuevo método permitió estudiar el efecto inhibitorio de la galotanina, producto natural de especies del género Rhus (Rhus chinensis),en la actividad de la LbNMNAT.spa
dc.description.abstractThe enzyme nicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT, EC 2.7.7.1/18) plays a central role in the nicotinamide and adenine dinucleotide (NAD) synthesis as its de novo and salvage pathways converge in it. NAD is a crucial molecule in the metabolism of all living beings, mainly in redox metabolism. Here we describe a new methodological strategy for the evaluation of possible inhibitors of Leishmania braziliensis NMNAT (LbNMNAT). Our method suppresses the cross inhibitory activity of the enzyme alcohol dehydrogenase (ADH, EC 1.1.1.1) coupled to the detection assay. We improved the specificity of the coupled enzymatic system, by introducing an additional solid-phase extraction step of the inhibitors prior to the execution of the coupled detection system. This step enabled the differentiated evaluation of the LbNMNAT activity without interfering in the coupled ADH enzyme, as well as the assessment of the inhibitory activity of gallotannin, an inhibitor found in Rhus chinensis, on LbNMNAT.eng
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherAcademia Colombiana de Ciencias Exactas, Fisicas y Naturalesspa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titleOptimización del ensayo de inhibición de nicotinamida/nicotinato mononucleótido adenililtransferasa de Leishmania braziliensisspa
dc.titleOptimization of the nicotinamide/nicotinate mononucleotide adenylyltransferase inhibition assay from Leishmania braziliensiseng
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad cientificaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.versioninfo:eu-repo/semantics/updatedVersionspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.identifier.doihttps://doi.org/10.18257/raccefyn.1272-
dc.subject.proposalEnsayo de actividadspa
dc.subject.proposalActivity assayeng
dc.subject.proposalNicotinamida/mononucleótido de nicotinato adenililtransferasa (NMNAT)spa
dc.subject.proposalNicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT) | Enzyme inhibitorseng
dc.subject.proposalLeishmaniaspa
dc.subject.proposalLeishmaniaeng
dc.subject.proposalgalotaninospa
dc.subject.proposalGallotannineng
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Fisicas y Naturalesspa
dc.relation.citationvolume45spa
dc.relation.citationstartpage721spa
dc.relation.citationendpage730spa
dc.contributor.corporatenameAcademia Colombiana de Ciencias Exactas, Fisicas y Naturalesspa
dc.identifier.eissn2382-4980spa
dc.relation.citationissue176spa
dc.type.contentTextspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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