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dc.contributor.authorTorres Sáez, Rodrigo G.-
dc.description.abstractEn este trabajo, se llevó a cabo modificaciones químicas de preparaciones de lipasa B de Candida antarctica (CALB) inmovilizadas en soportes de octil-agarosa, agarosa-Bromuro Cianógeno-(BrCN) y Eupergit C usando diferentes compuestos químicos, por ej. Etilendiamina (EDA), anhídrido succínico (SA) y ácido 2,4,6-trinitrobenceno-sulfónico (TNBS). Estas modificaciones de la superficie de la enzima causó cambios en las propiedades tales como carga neta (punto isoeléctrico o balance de grupos catiónicos/aniónicos) o hidrofobicidad (solubilidad), y demostraron ser métodos prácticos para mejorar el funcionamiento del biocatalizador (estabilidad, actividad y enantioselectividad). Estas alteraciones en las propiedades de la enzima por modificación química podría ser debida a cambios en la estructura de la forma activa de CALB. De esta manera, la modificación química en fase sólida de lipasas inmovilizadas podría convertirse en una herramienta poderosa en el diseño de librerías de lipasas con propiedades muy
dc.description.abstractIn this work, it was carried out chemical modifications of Candida antarctica lipase B (CALB) preparations immobilized on octyl-agarose, BrCN-agarose and Eupergit-C supports using different chemical compounds, e.g. ethylenediamine (EDA), succinic anhydride (SA) and 2,4,6-trinitrobenzensulfonic acid (TNBS). These modifications of the enzyme surface caused changes in physical properties such as net charge (isoelectric point or balance of cationic/anionic groups) or hydrophobicity (solubility), and proved to be practical methods to enhance the biocatalyst performance (stability, activity and enantio-selectivity). These alterations in enzyme properties by chemical modification should be due to changes in the structure of the active form of CALB. Therefore, solid phase chemical modification of immobilized lipases may become a powerful tool in the design of lipase libraries with very different properties.eng
dc.format.extent24 páginasspa
dc.publisherAcademia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internationalspa
dc.titleVol. 38 Suplemento Modificación Química en Fase Sólida de Lipasa B de Candida antarctica para mejorar sus propiedades de actividad, estabilidad y enantioselectividadspa
dc.typeArtículo de revistaspa
dcterms.audienceEstudiantes, Profesores, Comunidad científicaspa
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dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.subject.proposalCandida antarctica Bspa
dc.subject.proposalCandida antarctica Beng
dc.subject.proposalModificación químicaspa
dc.subject.proposalChemical modificationeng
dc.relation.ispartofjournalRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturalesspa
dc.publisher.placeBogotá, Colombiaspa
dc.contributor.corporatenameUniversidad Industrial de Santander, Bucaramanga, Santander, Colombiaspa
Appears in Collections:BA. Revista de la Academia Colombiana de Ciencias Exactas Físicas y Naturales

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