TY - GEN 
TI - Electrophoretic Karyotype of Clinical Isolates of Paracoccidioides brasiliensis
AU - Montoya, Astrid E.
AU - Moreno, Maria Neifer
AU - Restrepo Moreno, Ángela
AU - McEwen, Juan Guillermo
AB - Chromosomal DNA molecules were obtained from eight clinical isolates ofParacoccidioides brasiliensisduring the early stages of the yeast to mycelial transformation process and were separated by contour-clamped homogeneous electric field gel electrophoresis. In seven of the isolates, it was possible to separate five bands, albeit with two different migration patterns (A and B). In the remaining isolate only four bands were observed (pattern C). The isolated chromosomes showed molecular sizes ranging from 3.2 to 10 Mb according toSchizosaccharomyces pombemolecular size standards. The three different patterns observed had bands of 3.2, 3.8, 4.1, 5.2, 6.7, 7.2, 8.8, and 10 Mb. These data imply that at least 30 Mb of the genome are organized as chromosomal macromolecules. Due to the fact thatP. brasiliensisspheroplasts are difficult to obtain, technical details are provided.
DA - 1997
KW - Hongos - Experimentos
PB - Elsevier
UR - https://repositorio.accefyn.org.co/handle/001/1049
ER -