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Title: | lndirect fluorescent-antibody and quantitative agar-gel lmmunodiffusion tests for the serological diagnosis of paracoccidioidomycosis |
Authors: | Restrepo Moreno, Ángela Moncada F., Luz H. |
Issue Date: | 1972 |
Publisher: | American Society for Microbiology |
Abstract: | The value of various serological tests in the diagnosis of paracoccidioidomycosis was studied. Quantitative agar-gel immunodiffusion and indirect immunofluorescent tests were performed, and the results were compared with those of complement fixation and qualitative agar-gel procedures. The quantitative immunodiffusion procedure was found to serve as the simplest and safest quantitative test that could be performed for evaluation purposes, whereas the indirect fluorescent-antibody test gave nonspecific reactions and, as such, proved unsuitable. Se estudió el valor de diversas pruebas serológicas en el diagnóstico de paracoccidioidomicosis. Se realizaron pruebas cuantitativas de inmunodifusión en gel de agar e inmunofluorescencia indirecta y se compararon los resultados con los de la fijación del complemento y los procedimientos cualitativos de gel de agar. Se descubrió que el procedimiento de inmunodifusión cuantitativa sirve como la prueba cuantitativa más simple y segura que se puede realizar con fines de evaluación, mientras que la prueba de anticuerpos fluorescentes indirectos dio reacciones inespecíficas y, como tal, resultó inadecuada. |
URI: | https://repositorio.accefyn.org.co/handle/001/1056 |
ISSN: | 0003-6919 |
Document url: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC380559/ |
DOI: | 10.1128/am.24.1.132-137.1972 |
Cite Source: | Restrepo M., Ángela & Moncada F., Luz H. (1972). lndirect fluorescent-antibody and quantitative agar-gel lmmunodiffusion tests for the serological diagnosis of paracoccidioidomycosis. Applied Microbiology, 24(1):132-137 |
Appears in Collections: | DHAC Paracoccidioidomicosis. Diagnóstico |
Files in This Item:
File | Description | Size | Format | |
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indirect fluorescent antibody.pdf | 5.1 MB | Adobe PDF | View/Open |
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